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Anfahrt




Custom Mouse Monoclonal Antibody Production

Antibody availability is important for research needs. We offer monoclonal antibody development in mice with guaranteed success, where success is defined as the availability of positive stable clones from each target molecule against the immunogen used in the Dot Blot/ELISA and/or Western Blot assays. Customers have the option to provide fusion protein, mammalian cell or tissue lysate for antibody screening.    

General Working Progress of Monoclonal Antibody Development

  • Evaluation of Standard Antibody Production
  • Service Pre-Production
  • Discussion: Custom Specification, Flowchart,
  • Cost Purchase Order
  • Production Specification Approval
  • Service Agreement
  • Monoclonal Antibody Development and Progress Report
  • Monoclonal Hybridoma and Supernatant Delivery

Service 1: In Vitro Expressed Protein Immunization

  • Mab Production Specification Template (PDF, Excel File)
  • Production Flowchart  (graphical view)
  • Duration: 4 Months Service Agreement
  • Total Price:(INQUIRE)

Service 2: Protein Immunization

  • Mab Production Specification Template (PDF, Excel File)
  • Production Flowchart (graphical view)
  • Duration: 3 Months
  • Service Agreement
  • Total Price: (INQUIRE)

Service 3: Peptide Immunization

  • Mab Production
  • Specification Template  (PDF, Excel File)
  • Production Flowchart  (graphical view)
  • Duration: 3 Months Service
  • Agreement Total Price: (INQUIRE)


Phosphospecific Antibody


  • Phosphopeptide synthesis: 15mg purified (>90%, upto 15 mer amino acid residues.
  • ---phosphospecific epitope affinity purification: 5mg
  • ---KLH conjugation used for antibody production: 4mg
  • ---BSA conjugation: 1mg
  • ---Delivery to customer: 5mg
  • Non-phosphopeptide synthesis: 11mg purified (>80%, up to 15 mer amino acid residues)
  • ---Affinity depletion: 5mg
  • ---BSA conjugation : 1mg
  • ---Delivery to customer: 5mg
  • Immunization: 2 rabbits
  • Phosphopeptide and non-phosphopeptide ELISA titration of antibody for all bleeds
  • Pre-immune sample of serum (5ml/each rabbit) and anti-serum (100ml total)
  • Affinity purification of serum
  • Depletion of nonphosphospecific peptide matrix
  • Shipment of purified phosphospecific antibody (confirmed by ELISA) 



Ask for detailed information: info@normae.de