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Code No.DescriptionSpeciesFormatLink
ADE-2001Adenovirus Antigen Rapid TestHuman6 Tests
ADE-2002Adenovirus Antigen Rapid TestHuman24 Tests
AR-001Adeno/Rota Duo Antigen Rapid TestHuman6 Tests
AR-002Adeno/Rota Duo Antigen Rapid TestHuman24 Tests


Adeno One- Step Antigen

Cat.No. ADE-2001, 2002

For detection of the Adeno virus in faeces samples and tissue cultures.

Introduction

  • Adeno virus was discovered in 1953. The Adeno virus causes pharyngitis/bronchitis/-pneumonia.
  • 15% of respiration diseases in children under 5 years of age are caused by Adeno virus. 11% of the acute gastro-enteritis cases are caused by the virus. Ocular diseases (Keratoconjunctivitis) swimming pool conjunctivitis can occur at all different ages, especially in immune suppressed people (SLE, HIV etc.).
  • Epidemics of enteric Adeno have been found several times combined with Adeno virus infections. Cultivation of the Adeno virus can be very difficult, especially the enteric types; it can take sometimes 28 days before the virus can be detected in the culture.
  • There are 4 Adeno virus groups. Group I and II are both from the non enteric Adeno group. Group III (type 41) and group IV (type 40) are both enteric subtypes. The Adeno One- Step Test detects group specific Adeno virus antigen in samples of all kind of species (human, mice, rind, pig, etc.)

Principle of the test

  • The Adeno clinic test kit is based on a chromatographic test strip and two monoclonal antibodies to Adeno virus antigen.
  • One of the monoclonal antibodies is conjugated to the gold particles and the other is immobilised on the strip in the test zone. Adeno virus present in a test sample applied to the test strip will bind to the gold particles which then migrate to the test zone.
  • Also immobilised on the strip in the control zone is an Adeno virus antigen which binds to the gold bound monoclonal antibodies to indicate that the test is working properly.

Test components

  • 6 x test strips (only open the pouch just before use)
  • 6 x cotton swabs
  • 6 x pipettes
  • 6 x vials containing 600 m l buffer
  • 1 x pack insert

Test procedure

  • Take a new swab for each sample.
  • Take a small sample of faeces or a rectal swab using the included swab.
  • Wash the swab in the buffer vial. (see fig 1.)
  • Let clots of faeces sink to the bottom. (see fig 2.)
  • Unpack the strip.
  • Add 4 drops of the sample solution with the pipette to the strip. (see fig 3.)
  • Read the result after 5 to 15 minutes.



Interpretation of the result

  • Positive: two bands are visible, in zone 'C' and in zone 'T' Þ the sample contains
    Adeno antigen. The patient is infected with Adeno virus. (fig. A)
  • Negative: only one band is visible in zone 'C' Þ the sample does not contain Adeno antigen.
    The patient is not infected. (fig. B)
  • Not Valid: no bands are visible, repeat the test procedure



Notes

  • A negative result, can still imply a very weak infection, in this case there are not
    enough virus particles per ml faeces to get a positive signal < 104/ml, but this is rare; infected ill human shed always > 10 8/ml.
  • Also incorrect sampling and/or storage can cause false negative results.
  • In case of respirator Adeno virus infection, it is better to test throat samples, because these will contain much more virus particles than the faeces samples.
  • Faeces samples containing staphylococcus aureus (Ecoli strains) with high protein A/G. Concentrations can result in false positive results. These infections are very rare in children

Storage

  • The tests need to be stored at room temperature in a sealed package.

Warning

  • Positive samples should be considered as infectious.

References

  • Brand et all Am I epidermal 90 484-500 1969
  • Fay et all Arch Environ Health 17 795-802 1968
  • De Jong Lancet 1 1298-1296 (1983)
  • Hermann et all Arch Virology 94 259-265 (1987)