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The Real-Time Diagnostic Kit for Avian Influenza Virus Subtypes (H5, H7, H9)
Description
This real-time diagnostic kit utilizes a primer-pair and a fluorescent labeled probe both specific for avian influenza virus (AIV ) H5 subtypes (H5, H7, H9) to amplify a target region of the HA gene on AIV genomic RNA he target RNA sequence, a 94 base-pair segment s, is a conservative region in the center of the HA gene. The probe, labeled with FAM at 5' end and MGB at 3' end, was designed to specifically bind to a certain part of the amplified region that is conservative to the AIV H5 subtype but exclusive of other AIV subtypes to effectively avoid false positives and false negatives. The changing fluorescence signal in the process can be recorded by on-line fluorescent detectors, amplifying and detecting the target DNA sequence in real time.
This kit is optimized to detect AIV subtypes specifically and sensitively using samples of blood, poultry products, allantoic fluid, or cotton swabs.
Kit components
Component (48 Tests/kit)
Specification
Sample preparation reagent
- Lysis solution (15ml)
Nucleic acid amplification reagent
- AIV Gene reaction solution (700µl)
- RTase (25µl)
- Ampliase(25µl)
- DEPC-treated double-distilled water (ddW) (1ml)
General Control
- Negative control (1ml); Positive control (1ml)
Storage conditions and expiry date
- Store lysis solution at 4°C and other components at -20°C
- The kit will expire in 6 months. Make sure to use before the date of expiration.
Additional materials required
Reagents (AP)
- 100% Isopropanol (2-propanol) pre-frozen at -20°C.
- 75% (V/V) ethanol prepared with RNase- or DNase-free water stored at room temperature.
Applicable instruments
- Roche Lightcycler, MJ Opticon, Bio-Rad, PE Gene Amp 5700, PE Gene Amp 7700, ABI PRISM 7000, 7900, 7300, 7500, BIOR Line-Gene, Roter Gene, or other real-time PCR detection system.
Figure 1. Detection of influenza H5 and H7 subtypes by a fluorescent labeled probe
For more details or ordering, please contact DR.WANG, Fax:+49 7071 792022, E-Mail: info@normae.de
